Richard Twyman's Publications
Review articles
Kouhen M, García-Caparrós P, Twyman RM, Abdelly C, Mahmoudi H, Schillberg S, Debez A (2023) Improving environmental stress resilience in crops by genome editing: insights from extremophile plants. Critical Reviews in Biotechnology 43 (4) 559–574. DOI
Gurke R, Bendes A, Bowes J, Koehm M, Twyman RM, Barton A, Elewaut D, Goodyear C, Hahnefeld L, Hillenbrand R, Hunter E, Ibberson M, Ioannidis V, Kugler S, Lories RJ, Resch E, Rüping S, Scholich K, Schwenk JM, Waddington JC, Whitfield P, Geisslinger G, FitzGerald O, Behrens F, Pennington S (2022) OMICS and multi‐OMICS analysis for the early identification and improved outcome of patients with psoriatic arthritis. Biomedicines 10 (10) 2387. DOI
Hundleby PAC, D’Aoust MA, Finkle C, Atkins J, Twyman RM (2022) Regulation of molecular farming products. Methods in Molecular Biology 2480, 313–333. DOI
He W, Baysal C, Lobato Gómez M, Huang X, Alvarez D, Zhu C, Armario-Najera V, Blanco Perera A, Cerda Bennaser P, Saba-Mayoral A, Sobrino-Mengual G, Vargheese A, Abranches R, Abreu IA, Balamurugan S, Bock R, Buyel JF, Cunha NBD, Daniell H, Faller R, Folgado A, Gowtham I, Häkkinen ST, Shashi K, Ramalingam SK, Lacorte C, Lomonossoff GP, Luís IM, K-C Ma J, McDonald KA, Murad A, Nandi S, O'Keefe B, Parthiban S, Paul MJ, Ponndorf D, Rech E, Rodrigues JCM, Ruf S, Schillberg S, Schwestka J, Shah PS, Singh R, Stoger E, Twyman RM, Varghese IP, Vianna GR, Webster G, Wilbers RHP, Christou P, Oksman-Caldentey KM, Capell T (2021) Contributions of the international plant science community to the fight against infectious diseases in humans - part 2: affordable drugs in edible plants for endemic and re-emerging diseases. Plant Biotechnology Journal 19 (10) 1921–1936. DOI
Lobato Gómez M, Huang X, Alvarez D, He W, Baysal C, Zhu C, Armario-Najera V, Blanco Perera A, Cerda Bennasser P, Saba-Mayoral A, Sobrino-Mengual G, Vargheese A, Abranches R, Abreu IA, Balamurugan S, Bock R, Buyel JF, da Cunha NB, Daniell H, Faller R, Folgado A, Gowtham I, Häkkinen S, Shashi K, Kumar Ramalingam S, Lacorte C, Lomonossoff GP, Luís IM, Ma JK, McDonald KA, Murad A, Nandi S, O'Keefe B, Oksman-Caldentey KM, Parthiban S, Paul M, Ponndorf D, Rech E, Rodrigues JCM, Ruf S, Schillberg S, Schwestka J, Shah PS, Singh R, Stoger E, Twyman RM, Varghese IP, Vianna GR, Webster G, Wilbers RHP, Capell T, Christou P (2021) Contributions of the international plant science community to the fight against human infectious diseases - part 1: epidemic and pandemic diseases. Plant Biotechnology Journal 19 (10) 1901–1920. DOI
Capell T, Twyman RM, Armario-Najera V, Ma JK-C, Schillberg S, Christou P (2020) Potential applications of plant biotechnology against SARS-CoV-2. Trends in Plant Science 25 (7) 635–643. DOI
Girón-Calva PS, Twyman RM, Albajes R, Gatehouse AMR, Christou P (2020) The impact of environmental stress on Bt crop performance. Trends in Plant Science 25 (3) 264–278. DOI
Webb SR, Twyman RM, Moloney M (2020) Agtech infrastructure for pandemic preparedness. Nature Biotechnology 38, 1025–1027. DOI
Armario Najera V, Twyman RM, Christou P, Zhu C (2019) Applications of multiplex genome editing in higher plants. Current Opinion in Biotechnology 59, 93–102. DOI
Fischer R, Holland T, Sack M, Schillberg S, Stoger E, Twyman RM, Buyel JF (2018) Glyco-engineering of plant-based expression systems. Advances in Biochemical Engineering/Biotechnology 175, 137–166. DOI
Zhu C, Farré G, Zanga D, Lloveras J, Michelena A, Ferrio JP, Voltas J, Slafer G, Savin R, Albajes R, Eizaguirre M, Lopez C, Cantero-Martínez C, Díaz-Gómez J, Nogareda C, Moreno JA, Angulo E, Estany J, Pena RN, Tor M, Portero-Otin M, Eritja N, Arjó G, Serrano JCE, Matias-Guiu X, Twyman RM, Sandmann G, Capell T, Christou P (2018) High-carotenoid maize: development of plant biotechnology prototypes for human and animal health and nutrition. Phytochemistry Reviews 17 (2) 195–209. DOI
Buyel JF, Twyman RM, Fischer R (2017) Very-large-scale production of antibodies in plants: the biologization of manufacturing. Biotechnology Advances 35 (4) 458–465. DOI
Díaz-Gómez J, Twyman RM, Zhu C, Farré G, Serrano JCE, Portero-Otin M, Muñoz P, Sandmann G, Capell T, Christou P (2017) Biofortification of crops with nutrients: factors affecting utilization and storage. Current Opinion in Biotechnology 44, 115–123. DOI
Edgue G, Twyman RM, Beiss V, Fischer R, Sack M (2017) Antibodies from plants for bionanomaterials. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology 9 (6) e1462. DOI
Zhu C, Bortesi L, Baysal C, Twyman RM, Fischer R, Capell T, Schillberg S, Christou P (2017) Characteristics of genome editing mutations in cereal crops. Trends in Plant Science 22 (1) 38–52. DOI
Boes A, Reimann A, Twyman RM, Fischer R, Schillberg S, Spiegel H (2016) A plant-based transient expression system for the rapid production of malaria vaccine candidates. Methods in Molecular Biology 1404, 597–619. DOI
Bortesi L, Zhu C, Zischewski J, Perez L, Bassié L, Nadi R, Forni G, Lade S, Soto E, Jin X, Medina V, Villorbina G, Munoz P, Farré G, Fischer R, Twyman RM, Capell T, Christou P, Schillberg S (2016) Patterns of CRISPR/Cas9 activity in plants, animals and microbes. Plant Biotechnology Journal 14 (12) 2203–2216. DOI
Lee KL, Twyman RM, Fiering S, Steinmetz NF (2016) Virus-based nanoparticles as platform technologies for modern vaccines. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology 8 (4) 544–578. DOI
Berman J, Zorrilla-López U, Farré G, Zhu C, Sandmann G, Twyman RM, Capell T, Christou P (2015) Nutritionally important carotenoids as consumer products. Phytochemistry Reviews 14 (5) 727–743. DOI
Buyel JF, Fischer R, Twyman RM (2015) Extraction and downstream processing of plant-derived recombinant proteins. Biotechnology Advances 33 (6.1) 902–913. DOI
Farré G, Twyman RM, Christou P, Capell T, Zhu C (2015) Knowledge-driven approaches for engineering complex metabolic pathways in plants. Current Opinion in Biotechnology 32, 54–60. DOI
Fischer R, Vasilev N, Twyman RM, Schillberg S (2015) High-value products from plants: the challenges of process optimization. Current Opinion in Biotechnology 32, 156–162. DOI
Laibach N, Post J, Twyman RM, Schulze Gronover C, Prüfer D (2015) The characteristics and potential applications of structural lipid droplet proteins in plants. Journal of Biotechnology 201, 15–27. DOI
Mukherjee K, Twyman RM, Vilcinskas A (2015) Insects as models to study the epigenetic basis of disease. Progress in Biophysics and Molecular Biology 118 (1–2) 69–78. DOI
Fischer R, Buyel JF, Schillberg S, Twyman RM (2014) Molecular farming in plants: the long road to the market. Biotechnology in Agriculture and Forestry 68, 27–41. DOI
Miralpeix B, Sabalza M, Twyman RM, Christou P, Capell T (2014) Strategic patent analysis in plant biotechnology: terpenoid indole alkaloid metabolic engineering as a case study. Plant Biotechnology Journal 12 (2) 117–134. DOI
Salgado D, Fischer R, Schillberg S, Twyman RM, Rasche S (2014) Comparative evaluation of heterologous production systems for recombinant pulmonary surfactant protein D. Frontiers in Immunology 5, 623. DOI
Vamvaka E, Twyman RM, Christou P, Capell T (2014) Can plant biotechnology help to break the HIV–malaria link? Biotechnology Advances 32 (2) 575–582. DOI
Berman J, Zhu C, Pérez-Massot E, Arjó G, Zorrilla-López U, Masip G, Banakar R, Sanahuja G, Farré G, Miralpeix B, Bai C, Vamvaka E, Sabalza M, Twyman RM, Bassié L, Capell T, Christou P (2013) Can the world afford to ignore biotechnology solutions that address food insecurity? Plant Molecular Biology 83 (1–2) 5–19. DOI
Fischer R, Ostafe R, Twyman RM (2013) Cellulases from insects. Advances in Biochemical Engineering/Biotechnology 136, 51–64. DOI
Fischer R, Schillberg S, Buyel JF, Twyman RM (2013) Commercial aspects of pharmaceutical production in plants. Current Pharmaceutical Design 19 (31) 5471–5477. DOI
Masip G, Sabalza M, Pérez-Massot E, Banakar R, Cebrian D, Twyman RM, Capell T, Albajes R, Christou P (2013) Paradoxical EU agricultural policies on genetically engineered crops. Trends in Plant Science 18 (6) 312–324. DOI
Paul MJ, Teh AYH, Twyman RM, Ma JKC (2013) Target product selection – where can molecular pharming make the difference? Current Pharmaceutical Design 19 (31) 5478–5485. DOI
Pérez-Massot E, Banakar R, Gómez-Galera S, Zorrilla-López U, Sanahuja G, Arjó G, Miralpeix B, Vamvaka E, Farré G, Rivera SM, Dashevskaya S, Berman J, Sabalza M, Yuan D, Bai C, Bassie L, Twyman RM, Capell T, Christou P, Zhu C (2013) The contribution of transgenic plants to better health through improved nutrition: opportunities and constraints. Genes and Nutrition 8 (1) 29–41. DOI
Sanahuja G, Farré G, Berman J, Zorrilla-López U, Twyman RM, Capell T, Christou P, Zhu C (2013) A question of balance: achieving appropriate nutrient levels in biofortified staple crops. Nutrition Research Reviews 26 (2) 235–245. DOI
Schillberg S, Raven N, Fischer R, Twyman RM, Schiermeyer A (2013) Molecular farming of pharmaceutical proteins using plant suspension cell and tissue cultures. Current Pharmaceutical Design 19 (31) 5531–5542. DOI
Twyman RM, Schillberg S, Fischer R (2013) Optimizing the yield of recombinant pharmaceutical proteins in plants. Current Pharmaceutical Design 19 (31) 5486–5494. DOI
Zorrilla-López U, Masip G, Arjó G, Bai C, Banakar R, Bassie L, Berman J, Farré G, Miralpeix B, Pérez-Massot E, Sabalza M, Sanahuja G, Vamvaka E, Twyman RM, Christou P, Zhu C, Capell T (2013) Engineering metabolic pathways in plants by multigene transformation. The International Journal of Developmental Biology 57 (6–8) 565–576. DOI
Farré G, Naqvi S, Sanahuja G, Sanahuja G, Bai C, Zorrilla-López U, Rivera SM, Canela R, Sandman G, Twyman RM, Capell T, Zhu C, Christou P (2012) Combinatorial genetic transformation of cereals and the creation of metabolic libraries for the carotenoid pathway. Methods in Molecular Biology 847, 419–435. DOI
Fischer R, Schillberg S, Hellwig S, Twyman RM, Drossard J (2012) GMP issues for plant-derived recombinant proteins. Biotechnology Advances 30 (2) 434–439. DOI
Gómez-Galera S, Twyman RM, Sparrow PAC, Van Droogenbroek B, Capell T, Christou P (2012) Field trials and tribulations – making sense of the regulations for experimental field trials of transgenic crops in Europe. Plant Biotechnology Journal 10 (5) 511–523. DOI
Khan I, Twyman RM, Arcalis E, Stoger E (2012) Using storage organelles for the accumulation and encapsulation of recombinant proteins. Journal of Biotechnology 7 (9) 1099–1108. DOI
Lico C, Santi L, Twyman RM, Pezzotti M, Avesani L (2012) The use of plants for the production of human therapeutic peptides. Plant Cell Reports 31 (3) 439–451. DOI
Bai C, Twyman RM, Farré G, Sanahuja G, Christou P, Capell T, Zhu C (2011) A golden era – pro-vitamin A enhancement in diverse crops. In Vitro Cell and Developmental Biology – Plants 47 (2) 205–221. DOI
Farré G, Bai C, Twyman RM, Capell C, Christou P, Zhu C (2011) Nutritious crops producing multiple carotenoids – a metabolic balancing act. Trends in Plant Science 16 (10) 532–540. DOI
Farré G, Twyman RM, Zhu C, Capell T, Christou P (2011) Nutritionally enhanced crops and food security: scientific achievements versus political expediency. Current Opinion in Biotechnology 22 (2) 245–251. DOI
Naqvi S, Ramessar K, Farré G, Sabalza M, Miralpeix B, Twyman RM, Capell T, Christou P, Zhu C (2011) High-value products from transgenic maize. Biotechnology Advances 29 (1) 40–53. DOI
Noll GA, Müller B, Twyman RM, Prüfer D (2011) Native and artificial forisomes: functions and applications. Applied Microbiology and Biotechnology 89 (6) 1675–1682. DOI
Sabalza M, Miralpeix B, Twyman RM, Capell T, Christou P (2011) EU legitimizes GM crop exclusion zones. Nature Biotechnology 29 (4) 315–317. DOI
Safarnejad MR, Salehi-Jouzani GR, Tabatabaie M, Twyman RM, Schillberg S (2011) Antibody-mediated resistance against plant pathogens. Biotechnology Advances 29 (6) 961–971. DOI
Sanahuja G, Subhasappa RB, Twyman RM, Capell T, Christou P (2011) Bacillus thuringiensis – a century of research, development and commercial applications. Plant Biotechnology Journal 9 (3) 283–300. DOI
Yuan D, Bassie L, Sabalza M, Miralpeix B, Dashevskaya S, Farré G, Rivera SM, Subhasappa RB, Bai C, Sanahuja G, Arjó G, Avilla E, Zorrilla-López U, Ugido N, López A, Almacellas D, Zhu C, Capell T, Hahne G, Twyman RM, Christou P (2011) The potential impact of plant biotechnology on the Millennium Development Goals. Plant Cell Reports 30 (3) 249–265. DOI
Farré G, Ramessar K, Twyman RM, Capell T, Christou P (2010) The humanitarian impact of plant biotechnology: recent breakthroughs vs bottlenecks for adoption. Current Opinion in Plant Biology 13 (2) 219–225. DOI
Peremarti A, Twyman RM, Gómez-Galera S, Naqvi S, Farré G, Sabalza M, Miralpeix B, Dashevskaya S, Yuan D, Ramessar K, Christou P, Zhu C, Bassie L, Capell T (2010) Promoter diversity in multigene transformation. Plant Molecular Biology 73 (4–5) 363–378. DOI
Ramessar K, Capell T, Twyman RM, Christou P (2010) Going to ridiculous lengths – European coexistence regulations for GM crops. Nature Biotechnology 28 (2) 133–136. DOI
Ramessar K, Capell T, Twyman RM, Quemada H, Christou P (2009) Calling the tunes on transgenic crops – the case for regulatory harmony. Molecular Breeding 23 (1) 99–112. DOI
Sparrow PAC, Twyman RM (2009) Biosafety and risk assessment of plant-made pharmaceuticals. Methods in Molecular Biology 483, 341–353. DOI
Twyman RM, Ramessar K, Quemada H, Capell T, Christou P (2009) Plant biotechnology: the importance of being accurate. Trends in Biotechnology 27 (11) 609–612. DOI
Ramessar K, Capell T, Twyman RM, Quemada H, Christou P (2008) Trace and traceability – a call for regulatory harmony. Nature Biotechnology 26 (9) 975–978. DOI
Spök A, Twyman RM, Fischer R, Ma JKC, Sparrow PAC (2008) Evolution of a regulatory framework for pharmaceuticals derived from genetically modified plants. Trends in Biotechnology 26 (9) 506–517. DOI
Sparrow PAC, Irwin JA, Dale PJ, Twyman RM, Ma JKC (2007) Pharma-Planta: road testing the developing regulatory guidelines for plant-made pharmaceuticals. Transgenic Research 16 (2) 147–161. DOI
Yusibov V, Rabindran S, Commandeur U, Twyman RM, Fischer R (2006) The potential of plant virus vectors for vaccine production. Drugs in R&D 7 (4) 203–217. DOI
Schillberg S, Twyman RM, Fischer R (2005) Opportunities for recombinant antigen and antibody expression in transgenic plants – technology assessment. Vaccine 23 (15) 1764–1769. DOI
Ma JKC, Barros E, Bock R, Christou P, Dale PJ, Dix PJ, Fischer R, Irwin J, Mahoney R, Pezzotti M, Schillberg S, Sparrow P, Stoger E, Twyman RM (2005) Molecular farming for new drugs and vaccines. Current perspectives on the production of pharmaceuticals in transgenic plants. EMBO Reports 6 (7) 593–599. DOI
Ma JKC, Chikwamba R, Sparrow P, Fischer R, Mahoney R, Twyman RM (2005) Plant-derived pharmaceuticals – the road forward. Trends in Plant Science 10 (12) 580–585. DOI
Twyman RM, Schillberg S, Fischer R (2005) Transgenic plants in the biopharmaceutical market. Expert Opinion on Emerging Drugs 10 (1) 185–218. DOI
Christou P, Twyman RM (2004) The potential of genetically enhanced plants to address food insecurity. Nutrition Research Reviews 17 (1) 23–42. DOI
Fischer R, Stoger E, Schillberg S, Christou P, Twyman RM (2004) Plant-based production of biopharmaceuticals. Current Opinion in Plant Biology 7 (2) 152–158. DOI
Hellwig S, Drossard J, Twyman RM, Fischer R (2004) Plant cell cultures for the production of recombinant proteins. Nature Biotechnology 22 (11) 1415–1422. DOI
Stoger E, Schillberg S, Twyman RM, Fischer R, Christou P (2004) Antibody production in transgenic plants. Methods in Molecular Biology 248, 301–318. DOI
Twyman RM (2004) SNP discovery and typing technologies for pharmacogenomics. Current Topics in Medicinal Chemistry 4 (13) 1421–1429. DOI
Fischer R, Twyman RM, Schillberg S (2003) Production of antibodies in plants and their use for global health. Vaccine 21 (7–8) 820–825. DOI
Kohli A, Twyman RM, Abranches A, Wegel E, Shaw P, Christou P, Stoger E (2003) Transgene integration, organization and interaction in plants. Plant Molecular Biology 52 (2) 247–258. DOI
Twyman RM, Primrose SB (2003) Techniques patents for SNP genotyping. Pharmacogenomics 4 (1) 67–79. DOI
Twyman RM, Stoger E, Schillberg S, Christou P, Fischer R (2003) Molecular farming in plants: host systems and expression technology. Trends in Biotechnology 21 (12) 570–578. DOI
Stoger E, Sack M, Perrin Y, Vaquero C, Torres E, Twyman RM, Christou P, Fischer R (2002) Practical considerations for pharmaceutical antibody production in different crop systems. Molecular Breeding 9 (3) 149–158. DOI
Twyman RM, Stoger E, Kohli A, Capell T, Christou P (2002) Selectable and screenable markers for rice transformation. Molecular Methods of Plant Analysis 22, 1–17. DOI
Twyman RM, Stoger E, Kohli A, Christou P (2002) Foreign DNA: integration and expression in transgenic plants. Genetic Engineering: Principles and Methods 24, 107–136. DOI
Twyman RM, Jones EA (1995) The regulation of neuron-specific gene expression in the mammalian nervous system. Journal of Neurogenetics 10 (2) 67–101. DOI