Richard Twyman's Publications
Research articles
2025, 2026 and in press
Grundmann L, Zimmermann MM, Iordanidis C, Känel A, Schwarze A, Wiedmann DR, Muth J, Twyman RM, Prüfer D, Noll GA (2026) A mutant allele of the long-day floral activator NtFT5 is associated with the short-day-specific flowering of tobacco cultivar Maryland Mammoth. Communications Biology (in press)
Känel A, Roelfs KU, Wissing M, Lenzen B, Klein M, Twyman RM, Noll GA, Prüfer D (2026) Vernalization reveals distinct roles of FLOWERING LOCUS T homologs in floral transition of perennial Taraxacum koksaghyz. Plant Science 362, 112743. DOI
Akhgari A, Sulli M, Ramata-Stunda A, Häkkinen ST, Nohynek L, Salwinski A, Demurtas OC, Borodušķis M, Pajumo M, Twyman RM, Oksman-Caldentey KM, Diretto G, Rischer H (2025) Capsicum chinense cell cultures: a biotechnological platform for the sustainable production of bioactive metabolites for the cosmetics market. Biocatalysis and Agricultural Biotechnology 63, 103478. DOI
Müller B, Niephaus E, Eisenreich W, Bröker JN, Twyman RM, Prüfer D, Schulze Gronover C (2025) The cis-prenyltransferase protein family in Taraxacum koksaghyz. The Plant Journal 121 (3) e17233. DOI
Vogel H, Rügen N, Wielsch N, Twyman RM, Tonk-Rügen M, Vilcinskas A (2025) Molecular basis of the explosive defence response in the bombardier beetle Brachinus crepitans. Royal Society Open Science 12 (5) 241823. DOI
Wolters SM, Schwarz L, Khairat R, Sturm K, Müller B, van Deenen N, Twyman RM, Prüfer D, Schulze Gronover C (2025) TkSRPP3/4 interactors TkGGR1 and TkLIL3 link plastid-like organelles with isoprenoid metabolism in Taraxacum koksaghyz latex. Plant Cell Reports 44 (7) 155. DOI
2020–2024
Roelfs KU, Känel A, Twyman RM, Prüfer D, Schulze Gronover C (2024) Epigenetic variation in early and late flowering plants of the rubber-producing Russian dandelion Taraxacum koksaghyz provides insights into the regulation of flowering time. Scientific Reports 14 (1) 4283. DOI
Schmidt FJ, Grundmann L, Lahme M, Seidemann M, Schwarze A, Lichtenauer S, Twyman RM, Prüfer D, Noll GA (2024) COL2-dependent photoperiodic floral induction in Nicotiana sylvestris seems to be lost in the N. sylvestris × N. tomentosiformis hybrid N. tabacum. Frontiers in Plant Science 14, 1249879. DOI
Sobrino-Mengual G, Alvarez D, Twyman RM, Gerrish C, Fraser PD, Capell T, Christou P (2024) Activation of the native PHYTOENE SYNTHASE 1 promoter by modifying near-miss cis-acting elements induces carotenoid biosynthesis in embryogenic rice callus. Plant Cell Reports 43 (5) 118. DOI
Wolters SM, Laibach N, Riekötter J, Roelfs KU, Müller B, Eirich J, Twyman RM, Finkemeier I, Prüfer D, Schulze Gronover C (2024) The interaction networks of small rubber particle proteins in the latex of Taraxacum koksaghyz reveal diverse functions in stress responses and secondary metabolism. Frontiers in Plant Science 15, 1498737. DOI
Muñoz-Basagoiti J, Monteiro FLL, Krumpe LRH, Armario-Najera V, Shenoy SR, Perez-Zsolt D, Westgarth HJ, Villorbina G, Bomfim LM, Raïch-Regué D, Nogueras L, Henrich CJ, Gallemí M, Moreira FRR, Torres P, Wilson J, D’arc M, Marfil S, Herlinger AL, Pradenas E, Higa LM, Otin MP, Trinité B, Twyman RM, Capell T, Tanuri A, Blanco J, Useros NI, Rech EL, Christou P, O’Keefe BR (2023) Cyanovirin-N binds to select SARS-CoV2 Spike oligosaccharides outside of the receptor binding domain and blocks infection by SARS-CoV2. Proceedings of the National Academy of Sciences USA 120 (10) e2214561120. DOI
Riekötter J, Oklestkova J, Muth J, Twyman RM, Epping J (2023) Transcriptomic analysis of Chinese yam (Dioscorea polystachya Turcz.) variants indicates brassinosteroid involvement in tuber development. Frontiers in Nutrition 10, 1112793. DOI
Wolters SM, Benninghaus VA, Roelfs KU, van Deenen N, Twyman RM, Prüfer D, Schulze Gronover C (2023) Overexpression of a pseudo-etiolated-in-light-like protein in Taraxacum koksaghyz leads to a pale green phenotype and enables transcriptome-based network analysis of photomorphogenesis and isoprenoid biosynthesis. Frontiers in Plant Science 14, 1228961. DOI
Zhou XR, Beier S, Brilhaus D, Martins Rodrigues C, Mühlhaus T, von Suchodoletz D, Twyman RM, Usadel B, Kranz A (2023) DataPLAN: A web-based data management plan generator for the plant sciences. Data 8 (11) 159. DOI
Känel P, Noll GA, Schroedter K, Naffin E, Kronenberg J, Busswinkel F, Twyman RM, Klämbt C, Prüfer D (2022) The tobacco phosphatidylethanolamine-binding protein NtFT4 increases the lifespan of Drosophila melanogaster by interacting with the proteostasis network. Aging 14 (7) 2989–3029. DOI
Rose J, Brand I, Bilstein-Schloemer M, Jachimska B, Twyman RM, Prüfer D, Noll GA (2022) The Ca2+ response of a smart forisome protein is dependent on polymerization. Protein Science 31 (3) 602–612. DOI
Schnieder N, Känel A, Zimmermann M, Kriebs K, Witte A, Wrobel LS, Twyman RM, Prüfer D, Furch ACU, Noll GA (2022) So similar yet so different: the distinct contributions of extrafascicular and fascicular phloem to transport and exudation in cucumber plants. Journal of Plant Physiology 271, 153643. DOI
Wieghaus A, Roelfs KU, Twyman RM, Prüfer D, Schulze Gronover C (2022) Comparative transcriptome analysis in Taraxacum koksaghyz to identify genes that determine root volume and root length. Frontiers in Genetics 12, 784883. DOI
Kronenberg J, Schrödter K, Noll GA, Twyman RM, Prüfer D, Känel P (2021) The tobacco phosphatidylethanolamine-binding protein NtFT4 simultaneously improves vitality, growth and protein yield in human cells. Biotechnology and Bioengineering 118 (10) 3770–3786. DOI
Rose J, Müller B, Groscurth S, Giese J, Eirich J, Finkemeier I, Twyman RM, Prüfer D, Noll GA (2021) The functionality of plant mechanoproteins (forisomes) is dependent on the dual role of conserved cysteine residues. International Journal of Biological Macromolecules 193 (B) 1332–1339. DOI
Edgü G, Freund LJ, Hartje S, Tacke E, Hofferbert HR, Twyman RM, Noll GA, Muth J, Prüfer D (2020) Fast, precise and reliable methods for the on-site multiplex detection of potato viruses. International Journal of Molecular Sciences 21 (22) 8741. DOI
Rose J, Visser F, Müller B, Senft M, Groscurth S, Sicking KF, Twyman RM, Prüfer, Noll GA (2020) Identification and molecular analysis of interaction sites in the MtSEO-F1 protein involved in forisome assembly. International Journal of Biological Macromolecules 144, 603–614. DOI
Schmidt FJ, Zimmermann MM, Lichtenauer S, Wiedmann DR, Grundmann L, Muth J, Twyman RM, Prüfer D, Noll GA (2020) The major floral promoter NtFT5 in tobacco (Nicotiana tabacum) is a promising target for crop improvement. Frontiers in Plant Science 10, 1666. DOI
Preprints
Zimmermann MM, Grundmann L, Känel A, Schwarze A, Wiedmann DR, Muth J, Twyman RM, Prüfer D, Noll GA (2022) Unraveling the mystery behind the short-day-specific flowering of tobacco cultivar Maryland Mammoth. bioRxiv 2022.06.27.497884. DOI
2015–2019
Jin X, Bai C, Bassie L, Nogareda C, Romagosa I, Twyman RM, Christou P, Zhu C (2019) ZmPBF and ZmGAMYB transcription factors independently transactivate the promoter of the maize (Zea mays) β-carotene hydroxylase 2 gene. New Phytologist 222 (2) 793–804. DOI
Mir-Artigues P, Twyman RM, Alvarez D, Cerda P, Balcells M, Christou P, Capell T (2019) A simplified techno-economic model for the molecular pharming of antibodies. Biotechnology and Bioengineering 116 (10) 2526–2539. DOI
Beinecke FA, Grundmann L, Wiedmann DR, Schmidt FJ, Caesar AS, Zimmermann M, Lahme M, Twyman RM, Prüfer D, Noll GA (2018) The FT/FD-dependent initiation of flowering under long-day conditions in the day-neutral species Nicotiana tabacum originates from the facultative short-day ancestor N. tomentosiformis. The Plant Journal 96 (2) 329–342. DOI
Unland K, Pütter K, Vorwerk K, van Deenen N, Twyman RM, Prüfer D, Schulze Gronover C (2018) Functional characterization of squalene synthase and squalene epoxidase in Taraxacum koksaghyz. Plant Direct 2 (6) e00063. DOI
Vamvaka E, Farré G, Molinos-Albert LM, Evans A, Canela-Xandri A, Twyman RM, Carrillo J, Ordóñez RA, Shattock RJ, O’Keefe BR, Clotet B, Blanco J, Khush GS, Christou P, Capell T (2018) Unexpected synergistic HIV neutralization by a triple microbicide produced in rice endosperm. Proceedings of the National Academy of Sciences USA 115 (33) E7854–E7862. DOI
Moreno JA, Díaz-Gómez J, Nogareda C, Angulo E, Sandmann G, Portero-Otin M, Serrano JCE, Twyman RM, Capell T, Zhu C, Christou P (2016) The distribution of carotenoids in hens fed on biofortified maize is influenced by feed composition, absorption, resource allocation and storage. Scientific Reports 6, 35346. DOI
Vamvaka E, Twyman RM, Murad AM, Melnik S, Teh AYH, Arcalis E, Altmann F, Stoger E, Rech E, Ma JKC, Christou P, Capell T (2016) Rice endosperm produces an underglycosylated and potent form of the HIV-neutralizing monoclonal antibody 2G12. Plant Biotechnology Journal 14 (1) 97–108. DOI
Epping J, van Deenen N, Niephaus E, Stolze A, Fricke J, Huber C, Eisenreich W, Twyman RM, Prüfer D, Schulze Gronover C (2015) A rubber transferase activator is necessary for natural rubber biosynthesis in dandelion. Nature Plants 1, 15048. DOI
Laibach N, Hillebrand A, Twyman RM, Prüfer D, Schulze Gronover C (2015) Identification of a rubber elongation factor protein in Taraxacum brevicorniculatum which is localized on rubber particles and promotes rubber biosynthesis. The Plant Journal 82 (4) 609–620. DOI
Ma JKC, Drossard J, Lewis D, Altmann F, Boyle J, Christou P, Cole T, Dale P, van Dolleweerd CJ, Isitt V, Katinger D, Lobedan M, Mertens H, Paul MJ, Rademacher T, Sack M, Hundleby PAC, Stiegler G, Stoger E, Twyman RM, Vcelar B, Fischer R (2015) Regulatory approval and a first-in-human phase I clinical trial of a monoclonal antibody produced in transgenic tobacco plants. Plant Biotechnology Journal 13 (8) 1106–1120. DOI
Rahnamaeian M, Cytrynska M, Zdybicka-Barabas A, Dobslaff K, Twyman RM, Züchner T, Sadd B, Regoes R, Schmid-Hempel P, Vilcinskas A (2015) Insect antimicrobial peptides show potentiating functional interactions against Gram-negative bacteria. Proceedings of the Royal Society B: Biological Sciences 282 (1806) 20150293. DOI
Shukla S, Eber FJ, Nagarajan AS, DiFranco NA, Schmidt N, Wen AM, Eiben S, Twyman RM, Wege C, Steinmetz NF (2015) The impact of aspect ratio on the biodistribution and tumor homing of rigid soft-matter nanorods. Advanced Healthcare Materials 4 (6) 874–882. DOI
2010–2014
Groscurth S, Müller B, Vissier F, Blob B, Menzel M, Rüping BA, Twyman RM, Prüfer D, Noll GA (2014) Uncertain role of MtSEO-F3 in assembly of Medicago truncatula forisomes. Plant Signaling and Behavior 9 (9) e29581. DOI
Häkkinen ST, Raven N, Henquet M, Laukkanen ML, Anderlei T, Pitkänen JP, Twyman RM, Bosch D, Oksman-Caldentey KM, Schillberg S, Ritala A (2014) Molecular farming in tobacco hairy roots by triggering the secretion of a pharmaceutical antibody. Biotechnology and Bioengineering 111 (2) 336–346. DOI
Müller B, Groscurth S, Rüping BA, Twyman RM, Prüfer D, Noll GA (2014) Molecular and ultrastructural analysis of forisome subunits reveals the principles of forisome assembly. Annals of Botany 113 (7) 1121–1137. DOI
Post J, Eisenreich W, Huber C, Twyman RM, Prüfer D, Schulze Gronover C (2014) Establishment of an ex vivo laticifer cell suspension culture from Taraxacum brevicorniculatum as a production system for cis-isoprene. Journal of Molecular Catalysis B: Enzymatic 103, 85–93. DOI
Zielonka S, Ernst AM, Hawat S, Twyman RM, Prüfer D, Noll GA (2014) Characterization of five subgroups of the sieve element occlusion gene family in Glycine max reveals genes encoding non-forisome P-proteins, forisomes and forisome tails. Plant Molecular Biology 86 (1–2) 51–67. DOI
Fricke J, Hillebrand A, Twyman RM, Prüfer D, Gronover CS (2013) Abscisic acid-dependent regulation of small rubber particle protein gene expression in Taraxacum brevicorniculatum is mediated by TbbZIP1.1. Plant and Cell Physiology 54 (4) 448–464. DOI
Grünwald S, Stellzig J, Adam IV, Weber K, Binger S, Boll M, Knorr E, Twyman RM, Vilcinskas A, Wenzel U (2013) Longevity in the red flour beetle Tribolium castaneum is enhanced by broccoli and depends on nrf-2, jnk-1 and foxo-1 homologous genes. Genes and Nutrition 8 (5) 439–448. DOI
Jekat SB, Ernst AM, von Bohl A, Zielonka S, Twyman RM, Noll GA, Prüfer D (2013) P-proteins in Arabidopsis are heteromeric structures involved in rapid sieve tube sealing. Frontiers in Plant Science 4, 225. DOI
Bucsenez M, Rüping B, Behrens S, Twyman RM, Noll GA, Prüfer D (2012) Multiple cis-regulatory elements are involved in the complex regulation of the sieve element-specific MtSEO-F1 promoter from Medicago truncatula. Plant Biology 14 (5) 714–724. DOI
Ernst AM, Jekat SB, Zielonka S, Müller B, Neumann U, Rüping B, Twyman RM, Krzyzanek V, Prüfer D, Noll GA (2012) Sieve element occlusion (SEO) genes encode structural phloem proteins involved in wound sealing of the phloem. Proceedings of the National Academy of Sciences USA 109 (28) E1980–E1989. DOI
Groscurth S, Müller B, Schwan S, Menzel M, Diekstall F, Senft M, Kendall A, Kommor BA, Neumann U, Kalischuk M, Kawchuk LM, Krzyzanek V, Heilmann A, Stubbs G, Twyman RM, Prüfer D, Noll GA (2012) Artificial forisomes – an ideal model system to study the assembly and reactivity of a macromolecular plant mechanoprotein. Biomacromolecules 13 (10) 3076–3086. DOI
Harig L, Beinecke FA, Oltmanns J, Muth J, Müller O, Rüping B, Twyman RM, Fischer R, Prüfer D, Noll GA (2012) Proteins from the FLOWERING LOCUS T-like subclade of the PEBP family act antagonistically to regulate floral initiation in tobacco. The Plant Journal 72 (6) 908–921. DOI
Post JJ, van Deenen N, Fricke J, Kowalski N, Wurbs D, Schaller H, Eisenreich W, Huber C, Twyman RM, Prüfer D, Schulze Gronover C (2012) Laticifer specific cis-prenyltransferase silencing affects the rubber, triterpene and inulin content of Taraxacum brevicorniculatum. Plant Physiology 158 (3) 1406–1417. DOI
Noll GA, Müller B, Ernst AM, Rüping B, Groscurth S, Twyman RM, Kawchuk LM, Prüfer D (2011) Characteristics of artificial forisomes from plants and yeast. Bioengineered Bugs 2 (2) 111–114. DOI
Müller B, Noll GA, Ernst AM, Rüping B, Groscurth S, Twyman RM, Kawchuk LM, Prüfer D (2010) Recombinant artificial forisomes provide ample quantities of smart biomaterials for use in technical devices. Applied Microbiology and Biotechnology 88 (3) 689–698. DOI
Before 2010
Noll G, Rüping B, Ernst AM, Bucsenez M, Twyman RM, Fischer R, Prüfer D (2009) The promoters of forisome genes MtSEO2 and MtSEO3 direct gene expression to immature sieve elements in Medicago truncatula and Nicotiana tabacum. Plant Molecular Biology Reporter 27 (4) 526–533. DOI
Wahler D, Schulze Gronover C, Richter C, Foucu F, Twyman RM, Moerschbacher BM, Fischer R, Muth J, Prüfer D (2009) Polyphenoloxidase silencing affects latex coagulation in Taraxacum spp. Plant Physiology 151 (1) 334–346. DOI
Muth J, Hartje S, Twyman RM, Hofferbert H-R, Tacke E, Prüfer D (2008) Precision breeding for novel starch variants in potato. Plant Biotechnology Journal 6 (8) 576–584. DOI
Nickel H, Kawchuk L, Twyman RM, Zimmermann S, Junghans H, Winter S, Fischer R, Prüfer D (2008) Plantibody-mediated inhibition of the Potato leafroll virus P1 protein reduces virus accumulation. Virus Research 136 (1–2) 140–145. DOI
Agrawal PK, Kohli A, Twyman RM, Christou P (2005) Transformation of plants with multiple cassettes generates simple transgene integration patterns and high expression levels. Molecular Breeding 16 (3) 247–260. DOI
Fitter AH, Gilligan CA, Hollingworth K, Kleczkowski A, Twyman RM, Pitchford JW, the members of the NERC Soil Biodiversity Programme (2005) Biodiversity and ecosystem function in soil. Functional Ecology 19 (3) 369–377. DOI
Girardi CL, Corrent AR, Lucchetta L, Zanuzo MR, Zanatta J, Da Costa TS, Twyman RM, Nora FR, Nora L, Silva JA, Rombaldi CV (2005) Effect of ethylene, intermittent warming and controlled atmosphere on postharvest quality and the occurrence of wooliness in peach (Prunus persica cv. Chiripa) during cold storage. Postharvest Biology and Technology 38 (1) 25–33. DOI
Kohli A, Prynne MQ, Miro B, Pereira A, Twyman RM, Capell T, Christou P (2004) Dedifferentiation-mediated changes in transposition behavior make the Activator transposon an ideal tool for functional genomics in rice. Molecular Breeding 13 (2) 177–191. DOI
Silva JA, da Costa TS, Lucchetta L, Marini LJ, Zanuzo MR, Nora L, Nora FR, Twyman RM, Rombaldi CV (2004) Characterization of ripening behavior in transgenic melons expressing an antisense 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene from apple. Postharvest Biology and Technology 32 (3) 263–268. DOI
Tang K, Sun X, Hu Q, Wu A, Lin C-H, Lin H-J, Twyman RM, Christou P, Feng T (2001) Transgenic rice plants expressing the ferredoxin-like protein (AP1) from sweet pepper show enhanced resistance to Xanthomonas oryzae pv. oryzae. Plant Science 160 (5) 1035–1042. DOI
Torres E, Gonzalez-Melendi P, Stoger E, Shaw P, Twyman RM, Nicholson L, Vaquero C, Fischer R, Christou P, Perrin Y (2001) Native and artificial reticuloplasmins co-accumulate in distinct domains of the endoplasmic reticulum and in post-endoplasmic reticulum compartments. Plant Physiology 127 (3) 1212–1223. DOI
Fu X, Duc LT, Fontana S, Bong BB, Tinjuangjun P, Sudhakar D, Twyman RM, Christou P, Kohli A (2000) Linear transgene constructs lacking vector backbone sequences generate low-copy-number transgenic plants with simple integration patterns. Transgenic Research 9 (1) 11–19. DOI
Fu X, Kohli A, Twyman RM, Christou P (2000) Alternative silencing effects involve distinct types of non-spreading cytosine methylation at a three-gene single-copy transgenic locus in rice. Molecular and General Genetics 263 (1) 106–118. DOI
Kohli A, Griffiths S, Palacios N, Twyman RM, Vain P, Laurie DA, Christou P (1999) Molecular characterization of transforming plasmid rearrangements in transgenic rice reveals a recombination hotspot in the CaMV 35S promoter and confirms the predominance of microhomology mediated recombination. The Plant Journal 17 (6) 591–601. DOI
Twyman RM, Jones EA (1997) Sequences in the proximal 5' flanking region of the rat neuron-specific enolase (NSE) gene are sufficient for cell type-specific reporter gene expression. Journal of Molecular Neuroscience 8 (1) 63–73. DOI