Richard Twyman's Review Articles

 2017 and in press 

Buyel JF, Twyman RM, Fischer R (2017) Very-large-scale production of antibodies in plants: the biologization of manufacturing. 
Biotechnology Advances 35 (4) 458465.  DOI 

Díaz-Gómez J, Twyman RM, Zhu C, Farré G, Serrano JCE, Portero-Otin M, Muñoz P, Sandmann G, Capell T, Christou P (2017) Biofortification of crops with nutrients: factors affecting utilization and storage. 
Current Opinion in Biotechnolog44, 115123.  DOI 

Edgue G, Twyman RM, Beiss V, Fischer R, Sack M (2017) Antibodies from plants for bionanomaterials. 
Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology (online first).  DOI

Zhu C, Bortesi L, Baysal C, Twyman RM, Fischer R, Capell T, Schillberg S, Christou P (2017) Characteristics of genome editing mutations in cereal crops. 
Trends in Plant Science 22 (1) 3852.  DOI 

Zhu C, Farré G, Zanga D, Lloveras J, Michelena A, Ferrio JP, Voltas J, Slafer G, Savin R, Albajes R, Eizaguirre M, Lopez C, Cantero-Martínez C, Díaz-Gómez J, Nogareda C, Moreno JA, Angulo E, Estany J, Pena RN, Tor M, Portero-Otin M, Eritja N, Arjó G, Serrano JCE, Matias-Guiu X, Twyman RM, Sandmann G, Capell T, Christou P (2017) High-carotenoid maize: development of plant biotechnology prototypes for human and animal health and nutrition. 
Phytochemistry Reviews (online first).  DOI


Boes A, Reimann A, Twyman RM, Fischer R, Schillberg S, Spiegel H (2016) A plant-based transient expression system for the rapid production of malaria vaccine candidates. 
Methods in Molecular Biology 1404, 597–619.  DOI  

Bortesi L, Zhu C, Zischewski J, Perez L, Bassié L, Nadi R, Forni G, Lade S, Soto E, Jin X, Medina V, Villorbina G, Munoz P, Farré G, Fischer R, Twyman RM, Capell T, Christou P, Schillberg S (2016) Patterns of CRISPR/Cas9 activity in plants, animals and microbes.
Plant Biotechnology Journal 14 (12) 2203–2216.  DOI 

Lee KL, Twyman RM, Fiering S, Steinmetz NF (2016) 
Virus-based nanoparticles as platform technologies for modern vaccines. 
Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology 8 (4) 544–578.  DOI  


Berman J, Zorrilla-López U, Farré G, Zhu C, Sandmann G, Twyman RM, Capell T, Christou P (2015) Nutritionally important carotenoids as consumer products. 
Phytochemistry Reviews 14 (5) 727–743.  DOI  

Buyel JF, Fischer R, Twyman RM (2015) Extraction and downstream processing of plant-derived recombinant proteins.  
Biotechnology Advances 33 (6.1) 902–913.  DOI  

Farré G, Twyman RM, Christou P, Capell T, Zhu C (2015) Knowledge-driven approaches for engineering complex metabolic pathways in plants. 

Current Opinion in Biotechnology 32, 54–60.  DOI  

Fischer R, Vasilev N, Twyman RM, Schillberg S (2015) High-value products from plants: the challenges of process optimization. 

Current Opinion in Biotechnology 32, 156–162.  DOI   

Laibach N, Post J, Twyman RM, Schulze Gronover C, Prüfer D (2015) The characteristics and potential applications of structural lipid droplet proteins in plants. 

Journal of Biotechnology 201, 15–27.  DOI  

Mukherjee K, Twyman RM, Vilcinskas A (2015) Insects as models to study the epigenetic basis of disease. 

Progress in Biophysics and Molecular Biology 118 (1–2) 69–78.  DOI  


Fischer R, Buyel JF, Schillberg S, Twyman RM (2014) Molecular farming in plants: the long road to the market. 

Biotechnology in Agriculture and Forestry 68, 27–41.  DOI  

Miralpeix B, Sabalza M, Twyman RM, Christou P, Capell T (2014) Strategic patent analysis in plant biotechnology: terpenoid indole alkaloid metabolic engineering as a case study. 

Plant Biotechnology Journal 12 (2) 117–134.  DOI  

Salgado D, Fischer R, Schillberg S, Twyman RM, Rasche S (2014) Comparative evaluation of heterologous production systems for recombinant pulmonary surfactant protein D. 

Frontiers in Immunology 5, 623.  DOI  

Vamvaka E, Twyman RM, Christou P, Capell T (2014) Can plant biotechnology help to break the HIVmalaria link? 

Biotechnology Advances 32 (2) 575–582.  DOI  


Berman J, Zhu C, Pérez-Massot E, Arjó G, Zorrilla-López U, Masip G, Banakar R, Sanahuja G, Farré G, Miralpeix B, Bai C, Vamvaka E, Sabalza M, Twyman RM, Bassié L, Capell T, Christou P (2013) Can the world afford to ignore biotechnology solutions that address food insecurity? 

Plant Molecular Biology 83 (1–2) 5–19.  DOI  

Fischer R, Ostafe R, Twyman RM (2013) Cellulases from insects.

Advances in Biochemical Engineering/Biotechnology 136, 51–64.  DOI  

Fischer R, Schillberg S, Buyel JF, Twyman RM (2013) Commercial aspects of pharmaceutical production in plants. 

Current Pharmaceutical Design 19 (31) 5471–5477.  DOI  

Masip G, Sabalza M, Pérez-Massot E, Banakar R, Cebrian D, Twyman RM, Capell T, Albajes R, Christou P (2013) Paradoxical EU agricultural policies on genetically engineered crops. 

Trends in Plant Science 18 (6) 312–324.  DOI  

Paul MJ, Teh AYH, Twyman RM, Ma JKC (2013) Target product selection where can molecular pharming make the difference? 

Current Pharmaceutical Design 19 (31) 5478–5485.  DOI  

Pérez-Massot E, Banakar R, Gómez-Galera S, Zorrilla-López U, Sanahuja G, Arjó G, Miralpeix B, Vamvaka E, Farré G, Rivera SM, Dashevskaya S, Berman J, Sabalza M, Yuan D, Bai C, Bassie L, Twyman RM, Capell T, Christou P, Zhu C (2013) The contribution of transgenic plants to better health through improved nutrition: opportunities and constraints. 

Genes and Nutrition 8 (1) 29–41.  DOI  

Sanahuja G, Farré G, Berman J, Zorrilla-López U, Twyman RM, Capell T, Christou P, Zhu C (2013) A question of balance: achieving appropriate nutrient levels in biofortified staple crops. 

Nutrition Research Reviews 26 (2) 235–245.  DOI  

Schillberg S, Raven N, Fischer R, Twyman RM, Schiermeyer A (2013) Molecular farming of pharmaceutical proteins using plant suspension cell and tissue cultures. 

Current Pharmaceutical Design 19 (31) 5531–5542.  DOI  

Twyman RM, Schillberg S, Fischer R (2013) Optimizing the yield of recombinant pharmaceutical proteins in plants.  

Current Pharmaceutical Design 19 (31) 5486–5494.  DOI  

Zorrilla-López U, Masip G, Arjó G, Bai C, Banakar R, Bassie L, Berman J, Farré G, Miralpeix B, Pérez-Massot E, Sabalza M, Sanahuja G, Vamvaka E, Twyman RM, Christou P, Zhu C, Capell T (2013) Engineering metabolic pathways in plants by multigene transformation. 

The International Journal of Developmental Biology 57 (6–8) 565–576.  DOI  


Farré G, Naqvi S, Sanahuja G, Sanahuja G, Bai C, Zorrilla-López U, Rivera SM, Canela R, Sandman G, Twyman RMCapell T, Zhu C, Christou P (2012) Combinatorial genetic transformation of cereals and the creation of metabolic libraries for the carotenoid pathway. 

Methods in Molecular Biology 847, 419–435.  DOI  

Fischer R, Schillberg S, Hellwig S, Twyman RM, Drossard J (2012) GMP issues for plant-derived recombinant proteins. 

Biotechnology Advances 30 (2) 434–439.  DOI  


Gómez-Galera S, Twyman RM, Sparrow PAC, Van Droogenbroek B, Capell T, Christou P (2012) Field trials and tribulations making sense of the regulations for experimental field trials of transgenic crops in Europe. 

Plant Biotechnology Journal 10 (5) 511–523.  DOI  


Khan I, Twyman RM, Arcalis E, Stoger E (2012) Using storage organelles for the accumulation and encapsulation of recombinant proteins. 

Journal of Biotechnology 7 (9) 1099–1108.  DOI  


Lico C, Santi L, Twyman RM, Pezzotti M, Avesani L (2012) The use of plants for the production of human therapeutic peptides. 

Plant Cell Reports 31 (3) 439–451.  DOI  



Bai C, Twyman RM, Farré G, Sanahuja G, Christou P, Capell T, Zhu C (2011) A golden era pro-vitamin A enhancement in diverse crops. 

In Vitro Cell and Developmental Biology - Plants 47 (2) 205–221.  DOI  

Farré G, Bai C, Twyman RM, Capell C, Christou P, Zhu C (2011) 
Nutritious crops producing multiple carotenoids a metabolic balancing act. 

Trends in Plant Science 16 (10) 532–540.  DOI  

Farré G, Twyman RM, Zhu C, Capell T, Christou P (2011) 
Nutritionally enhanced crops and food security: scientific achievements versus political expediency. 

Current Opinion in Biotechnology 22 (2) 245–251.  DOI  

Naqvi S, Ramessar K, Farré G, Sabalza M, Miralpeix B, Twyman RM, Capell T, Christou P, Zhu C (2011) 
High value products from transgenic maize. 

Biotechnology Advances 29 (1) 40–53.  DOI  

Noll GA, Müller B,Twyman RM, Prüfer D (2011) 
Native and artificial forisomes: functions and applications. 

Applied Microbiology and Biotechnology 89 (6) 1675–1682.  DOI  

Sabalza M, Miralpeix B, Twyman RM, Capell T, Christou P (2011) 
EU legitimizes GM crop exclusion zones. 

Nature Biotechnology 29 (4) 315–317.  DOI  

Safarnejad MR, Salehi-Jouzani GR, Tabatabaie M, Twyman RM, Schillberg S (2011) 
Antibody-mediated resistance against plant pathogens. 

Biotechnology Advances 29 (6) 961–971.  DOI  

Sanahuja G, Subhasappa RB, Twyman RM, Capell T, Christou P (2011) 
Bacillus thuringiensis - a century of research, development and commercial applications. 

Plant Biotechnology Journal 9 (3) 283–300.  DOI  

Yuan D, Bassie L, Sabalza M, Miralpeix B, Dashevskaya S, Farré G, Rivera SM, Subhasappa RB, Bai C, Sanahuja G, Arjó G, Avilla E, Zorrilla-López U, Ugido N, López A, Almacellas D, Zhu C, Capell T, Hahne G, Twyman RM, Christou P (2011) 
The potential impact of plant biotechnology on the Millennium Development Goals. 

Plant Cell Reports 30 (3) 249–265.  DOI  

 2010 and earlier  

Farré G, Ramessar K, Twyman RM, Capell T, Christou P (2010) The humanitarian impact of plant biotechnology: recent breakthroughs vs bottlenecks for adoption. 

Current Opinion in Plant Biology 13 (2) 219–225.  DOI  

Peremarti A, Twyman RM, Gómez-Galera S, Naqvi S, Farré G, Sabalza M, Miralpeix B, Dashevskaya S, Yuan D, Ramessar K, Christou P, Zhu C, Bassie L, Capell T (2010) Promoter diversity in multigene transformation. 

Plant Molecular Biology 73 (45) 363378.  DOI  

Ramessar K, Capell T, Twyman RM, Christou P (2010) Going to ridiculous lengths European coexistence regulations for GM crops. 

Nature Biotechnology 28 (2) 133–136.  DOI  

Ramessar K, Capell T, Twyman RM, Quemada H, Christou P (2009) Calling the tunes on transgenic crops the case for regulatory harmony. 

Molecular Breeding 23 (1) 99–112.  DOI  

Sparrow PAC, Twyman RM (2009) Biosafety and risk assessment of plant-made pharmaceuticals. 

Methods in Molecular Biology 483, 341353.  DOI  

Twyman RM, Ramessar K, Quemada H, Capell T, Christou P (2009) Plant biotechnology: the importance of being accurate. 

Trends in Biotechnology 27 (11) 609–612.  DOI  

Ramessar K, Capell T, Twyman RM, Quemada H, Christou P (2008) Trace and traceability a call for regulatory harmony. 

Nature Biotechnology 26 (9) 975–978.  DOI  

Spök A, Twyman RM, Fischer R, Ma JKC, Sparrow PAC (2008) Evolution of a regulatory framework for pharmaceuticals derived from genetically modified plants. 

Trends in Biotechnology 26 (9) 506–517.  DOI  

Sparrow PAC, Irwin JA, Dale PJ, Twyman RM, Ma JKC (2007) Pharma-Planta: Road testing the developing regulatory guidelines for plant-made pharmaceuticals. 

Transgenic Research 16 (2) 147–161.  DOI  

Yusibov V, Rabindran S, Commandeur U, Twyman RM, Fischer R (2006) The potential of plant virus vectors for vaccine production. 

Drugs in R&D 7 (4) 203–217.  DOI  

Schillberg S, Twyman RM, Fischer R (2005) Opportunities for recombinant antigen and antibody expression in transgenic plants technology assessment. 

Vaccine 23 (15) 1764–1769.  DOI  

Ma JKC, Barros E, Bock R, Christou P, Dale PJ, Dix PJ, Fischer R, Irwin J, Mahoney R, Pezzotti M, Schillberg S, Sparrow P, Stoger E, Twyman RM (2005) Molecular farming for new drugs and vaccines. Current perspectives on the production of pharmaceuticals in transgenic plants. 

EMBO Reports 6 (7) 593–599.  DOI  

Ma JKC, Chikwamba R, Sparrow P, Fischer R, Mahoney R, Twyman RM (2005) Plant-derived pharmaceuticals the road forward. 

Trends in Plant Science 10 (12) 580–585.  DOI  

Twyman RM, Schillberg S, Fischer R (2005) Transgenic plants in the biopharmaceutical market. 

Expert Opinion on Emerging Drugs 10 (1) 185–218.  DOI  

Christou P, Twyman RM (2004) The potential of genetically enhanced plants to address food insecurity. 

Nutrition Research Reviews 17 (1) 23–42.  DOI  


Fischer R, Stoger E, Schillberg S, Christou P, Twyman RM (2004) Plant-based production of biopharmaceuticals. 

Current Opinion in Plant Biology 7 (2) 152–158.  DOI  

Hellwig S, Drossard J, Twyman RM, Fischer R (2004) Plant cell cultures for the production of recombinant proteins. 

Nature Biotechnology 22 (11) 1415–1422.  DOI  

Stoger E, Schillberg S, Twyman RM, Fischer R, Christou P (2004) Antibody production in transgenic plants. 

Methods in Molecular Biology 248, 301318.  DOI  

Twyman RM (2004) SNP discovery and typing technologies for pharmacogenomics. 

Current Topics in Medicinal Chemistry 4 (13) 1421–1429.  DOI  

Fischer R, Twyman RM, Schillberg S (2003) Production of antibodies in plants and their use for global health. 

Vaccine 21 (78) 820–825.  DOI  

Kohli A, Twyman RM, Abranches A, Wegel E, Shaw P, Christou P, Stoger E (2003) Transgene integration, organization and interaction in plants. 

Plant Molecular Biology 52 (2) 247–258.  DOI  

Twyman RM, Primrose SB (2003) Techniques patents for SNP genotyping. 

Pharmacogenomics 4 (1) 67–79.  DOI  

Twyman RM, Stoger E, Schillberg S, Christou P, Fischer R (2003) Molecular farming in plants: Host systems and expression technology. 

Trends in Biotechnology 21 (12) 570–578.  DOI  

Stoger E, Sack M, Perrin Y, Vaquero C, Torres E, Twyman RM, Christou P, Fischer R (2002) Practical considerations for pharmaceutical antibody production in different crop systems. 

Molecular Breeding 9 (3) 149–158.  DOI  

Twyman RM, Stoger E, Kohli A, Capell T, Christou P (2002) Selectable and screenable markers for rice transformation. 

Molecular Methods of Plant Analysis 22, 117.  DOI  


Twyman RM, Stoger E, Kohli A, Christou P (2002) Foreign DNA: integration and expression in transgenic plants. 

Genetic Engineering: Principles and Practice 24, 107136.  DOI  

Twyman RM, Jones EA (1995) The regulation of neuron-specific gene expression in the mammalian nervous system. 

Journal of Neurogenetics 10 (2) 67–101.  DOI  

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